Sarvesh Singh
King George’s Medical University India
Title: Association of NAT2 gene polymorphism with anti-tubercular drug induced hepatotoxicity in North Indian population
Biography
Biography: Sarvesh Singh
Abstract
Background: Tuberculosis (TB) is one of the important causes of global mortality and morbidity. Hepatotoxicity is a most serious adverse drug reaction of anti-TB drugs. Various genetic factors are associated with drug-induced hepatotoxicity (DIH). Anti-tubercular drugs are mostly metabolized by N-acetyltransferase 2 (NAT2). Therefore, in this study we aim to assess the association between of NAT2genotype polymorphism and drug-induced hepatotoxicity (DIH) in North India population.
Methods: TB patients were recruited in two groups. Seventy (70) TB patients were enrolled as tolerant control group who did not develop DIH, whereas 30 TB patients in anti-tubercular DIH group who developed liver injury during treatment. The genetic polymorphisms of the NAT2 genes were analyses by PCRRFLP. Genotype and allele frequencies were evaluated by t-test and odds ratio (OR) with 95% confidence intervals (CIs) to evaluate the strength of associations.
Results: There is high percentage of slow acetylators among North Indian population. The 4% people were fast acetylators, 34% were intermediate acetylators and 62% were slow acetylators. Patients with the slow acetylator genotypes were most common and there was no significant difference between DIH (73.33%) and non-DIH (61.40%) patients. However, the slow-acetylator genotypes (NAT2*6/7, NAT2*5/7 and NAT2*5/6) were also not significantly different in anti-tubercular DIH group and tolerant control group.
Conclusion: In present study, NAT2 genotype polymorphism was found to have no association with development of anti- tubercular DIH